ANXA1/Annexin A1 Antibody

SKU: orb1537970

Description

ANXA1/Annexin A1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (4 µg/ml), Peptide-ELISA (1:4000), WB (1 - 3 µg/ml)
• Reactivity: Bovine, Canine, Equine, Guinea pig, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kD according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al, Cancer Res. 2000 Nov15;60(22):6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence CQAILDETKGDYEK, from the C-Terminus of protein sequence according to NP_000691.1.
• Target: ANXA1 / Annexin A1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANXA1, Annexin A1, Annexin I, Annexin-1, ANX1, Calpactin II, Chromobindin-9, Lipocortin I, Annexin I (lipocortin I), Calpactin-2, LPC1

Goat Anti-Annexin I Antibody (1 µg/ml) staining of K562 (A) and NIH3T3 (B) lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence

Quick Database Links

Gene Symbol

ANXA1 / Annexin A1