Annexin A1 Antibody

• Catalog Number: orb1246601
• Category: Antibodies
• Description: Annexin A1 Antibody
• Target: ANXA1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: CQAILDETKGDYEK
• UniProt ID: P04083
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1). Recommended concentration: 1-3ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: annexin I, ANXA1, annexin A1, ANX1, LPC1, annexin
• Note: For research use only
• NCBI: NP_000691.1

orb1246601 (1 ug/ml) staining of K562 (A) and NIH3T3 (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

orb1246601 (1 ug/ml) staining of Rat Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246601 (1 ug/ml) staining of Pig Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246601 (4 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1246601 (4 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.