Annexin A1 Rabbit Polyclonal Antibody

• Catalog Number: orb10094
• Category: Antibodies
• Description: Annexin A1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Rabbit
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Annexin A1 (281-346/346aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: ANXA1
• UniProt ID: P04083
• RRID: AB_10748479
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Annexin I; AnnexinA1; AnnexinI; ANX 1; ANX A1; ANX
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Annexin A1) polyclonal Antibody, Unconjugated (orb10094) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Kidney (Rat) Lysate at 30 ug, Lane 2: Brain (Rat) Lysate at 30 ug, Primary: Anti-Annexin A1 (orb10094) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1: 3000 dilution, Predicted band size: 39kD, Observed band size: 40kD.

Tissue/cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Annexin A1 Polyclonal Antibody, Unconjugated (orb10094) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.