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Anti-WNT1 Antibody

Catalog Number: orb214739

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214739
CategoryAntibodies
DescriptionRabbit polyclonal antibody to WNT1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Porcine, Primate, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human WNT1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetWNT1
Entrez22408, 7471
UniProt IDP04628, P04426
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti INT1 antibody, anti Proto-oncogene Wnt-1 anti
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-WNT1 Antibody

Western blot analysis of WNT1 expression in HEK293T (A), SP2/0 (B), H9C2 (C), WI38 (D), NIH3T3 (E) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 40 kD)

Anti-WNT1 Antibody

Immunohistochemical analysis of WNT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-WNT1 Antibody

Immunofluorescent analysis of WNT1 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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