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Featured
Description
Research Area
Cell Biology
Images & Validation
−| Tested Applications | FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human E2F1 around the phosphorylation site of Ser332 IV(p-S)PP |
| Target | E2F1 |
| Molecular Weight | 61 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−E2F-1; RBAP1; RBBP3; RBP3; Tg(Wnt1-cre)2Sor; mKIAA4009; E2F1_HUMAN; E2F1; PBR3; Retinoblastoma-associated protein 1 (RBAP-1); Retinoblastoma-binding protein 3 (RBBP-3); pRB-binding protein E2F-1; E2F1_MOUSE; E2F1_RAT; E2F transcription factor 1; retinoblastoma-binding protein 3 (E2F-like); E2F1 (phospho-S332); p-E2F1; phospho-E2F1; E2F1 (phospho-Ser332)
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Quick Database Links
Gene Symbol
E2F1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence






