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| Catalog Number | orb547657 |
|---|---|
| Category | Antibodies |
| Description | Anti-Vinculin VCL Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat. |
| Clonality | Monoclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Purification | Affinity-chromatography |
| Immunogen | A synthesized peptide derived from human Vinculin |
| UniProt ID | P18206 |
| MW | 17 kDa |
| Tested applications | ICC, IF, IHC, IP, WB |
| Application notes | WB 1:10000-1:20000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Antibody Type | Primary Antibody |
| Clone Number | AIG-22 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Vinculin; Metavinculin; MV; VCL |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Western blot analysis of Vinculin expression in (1) HepG2 cell lysate; (2) HeLa cell lysate.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC, WB | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |