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Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody

Catalog Number: orb547658

DispatchCurrently estimated at 1-3 months
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Catalog Numberorb547658
CategoryAntibodies
DescriptionAnti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody
ClonalityMonoclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
PurificationAffinity-chromatography
ImmunogenA synthesized peptide derived from human RING2 / RING1B / RNF2 E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals.
UniProt IDQ99496
MW17 kDa
Tested applicationsFC, ICC, IF, IHC, WB
Application notesWB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Antibody TypePrimary Antibody
Clone NumberAEDC-18
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesVinculin; Metavinculin; MV; VCL
NoteFor research use only
Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody

Western blot analysis of RNF2 expression in HeLa cell lysate using anti-RNF2 antibody.