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| Catalog Number | orb704245 |
|---|---|
| Category | Antibodies |
| Description | Vinculin Recombinant Rabbit Monoclonal Antibody (Loading Control) |
| Target | VCL |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Purification | Affinity purified by Protein A |
| Immunogen | Recombinant Human Vinculin |
| UniProt ID | P18206 |
| MW | 124 kDa |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Clone Number | 5G7 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CMD1W; CMH15; Epididymis luminal protein 114; HEL1 Read more... |
| Research Area | Actin Binding Proteins, Actina, CARdiovascular Res Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human K562 cell lysates, Lane 5: Human HUVEC cell lysates, Primary: Anti-Vinculin (Loading Control) (orb704245) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124 kDa, Observed band size: 135 kDa.