Vinculin Rabbit Monoclonal Antibody

SKU: orb1474479

Description

The Vinculin Antibody is suitable for IF, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against A synthetic peptide of human Vinculin .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, IP, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Vinculin
• Target: VCL
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Vinculin; Metavinculin; MV

Western blot analysis of Vinculin expression in K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates. (Predicted band size: 124 kD; Observed band size: 124 kD)

Immunofluorescent analysis of Vinculin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

VCL

UniProt

P85972, Q64727, P18206

NCBI Gene ID

7414, 22330, 305679