VAT1 Antibody

SKU: orb668610

Description

The VAT1 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide of human VAT1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1-500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide of human VAT1. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Synaptic vesicle membrane protein VAT-1 homolog

Western blot analysis of VAT1 expression in K562 (A) whole cell lysates. (Predicted band size: 28; 34; 41 kD; Observed band size: 42 kD)

Immunofluorescent analysis of VAT1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q3MIE4, Q99536, Q62465

NCBI Gene ID

26949, 287721, 10493