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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 3.513888889, IHC: 1:500-1:5000, ICC: 1:500-1:2000 |
| Reactivity | Bovine, Human, Mouse, Porcine, Rat |
| Predicted Reactivity | Canine, Equine, Feline, Goat, Guinea pig, Hamster, Primate, Rabbit, Rodent, Sheep |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | BH7 |
| Immunogen | Recombinant full length human UCHL1 purified from E. coli. |
| Target | Ubiquitin C-terminal Hydrolase 1 (UCHL1) |
| Molecular Weight | 24 |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS + 50% glycerol and 5 mm NaN3 |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunofluorescence of a section of rat hippocampus showing specific labeling of UCHL1 (Cat. orb319704, 1:5000, green) in cell bodies and dendrites of neurons, and specific labeling of FOX3 (red). The blue is DAPI staining of nuclear DNA.

Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 (Cat. orb319704, 1:500, red) and anti-Neurofilament H (Cat. orb319585, 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.

Immunolabeling of HEK 293 cells labeled with anti-UCHL1 antibody (Cat. orb319704, green, 1:500) and anti-neuron specific enolase antibody (Cat. orb319615, red, 1:500). The blue is staining nuclear DNA.

Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.
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Ubiquitin C-terminal Hydrolase 1 (UCHL1) Antibody (orb319704)
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