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Anti-SOX2 Antibody

Catalog Number: orb411836

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb411836
CategoryAntibodies
DescriptionRabbit polyclonal antibody to SOX2
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide of human SOX2
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000, IHC-P: 1:50-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetSOX2
Entrez20674, 6657
UniProt IDP48432, P48431
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-Transcription factor SOX-2 antibody
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-SOX2 Antibody

Western blot analysis of SOX2 expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 35 kD)

Anti-SOX2 Antibody

Immunofluorescent analysis of SOX2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    Rabbit

    Polyclonal

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    100 μg
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    FC,  IF,  IH,  WB

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    Mouse

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    IF,  IH,  WB

    Human, Mouse, Rat

    Rabbit

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    200 μl, 100 μl, 50 μl
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    FC,  ICC,  WB

    Mouse

    Mouse

    Monoclonal

    Unconjugated

    100 μl