SMAD3 Antibody

• Catalog Number: orb1474609
• Category: Antibodies
• Description: The SMAD3 Antibody is suitable for IF, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against A synthetic peptide of human Smad3 .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: A synthetic peptide of human Smad3
• UniProt ID: P84025, P84022
• Tested applications: IF, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MADH3; Mothers against decapentaplegic homolog 3;
• Note: For research use only
• Entrez: 25631, 4088
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMAD3 expression in Jurkat (A), C6 (B), Hela (C) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 52 kD)

Immunohistochemical analysis of SMAD3 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.5). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of SMAD3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.