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Featured
Description
Research Area
Cancer Biology
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1ug/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | B5F3 |
| Immunogen | A synthesized peptide derived from human SMAD3 (200-250aa) |
| Target | SMAD3 |
| Molecular Weight | 50 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/1ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; hMAD-3; hSMAD3; mad3; Smad 3; SMAD3_HUMAN; SMAD3; MAD homolog 3; Mothers against DPP homolog 3; SMAD family member 3 (SMAD 3 | Smad3 | hSMAD3); SMAD3_MOUSE; mMad3; SMAD family member 3 (SMAD 3 | Smad3); SMAD3_RAT; SMAD family member 3; MAD, mothers against decapentaplegic homolog 3 (Drosophila); SMAD, mothers against DPP homolog 3 (Drosophila)
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Quick Database Links
Gene Symbol
SMAD3
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry











