Phospho-Smad3 (Ser423 + Ser425) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb526653
• Category: Antibodies
• Description: Phospho-Smad3 (Ser423 + Ser425) Recombinant Rabbit Monoclonal Antibody
• Target: SMAD3
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 CS(p-S)V(p-S)
• UniProt ID: P84022
• MW: 47 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Clone Number: 5C5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Smad3(phospho Ser423/425); Smad3(phospho S423+S425
• Research Area: Cancer Research, Cell Biology, Cell Signaling, Cyt
• Note: For research use only

Sample: Lung (Mouse) Lysate at 40 ug, A549 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Smad3 (Ser423 + Ser425) (orb526653) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 55 kD.