SEMA4D/CD100 Antibody

• Catalog Number: orb1806110
• Category: Antibodies
• Description: Anti-SEMA4D / CD100 Reference Antibody is expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Target: SEMA4D / CD100
• Clonality: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: Batch dependent
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Purity: >95%
• Purification: Protein A
• UniProt ID: Q92854
• MW: 145.88 kDa
• Tested applications: ELISA, FA, FACS, In vivo
• Expression System: CHO
• Endotoxins: <0.001 EU/ug
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-SEMA4D / CD100 Reference Antibody (pepinemab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-SEMA4D / CD100 Reference Antibody (pepinemab)is more than 100% ;determined by SEC-HPLC.

Immobilized human SEMA4D/CD100; His Tag at 2 μg/mL can bind Anti-SEMA4D / CD100 Reference Antibody (pepinemab);EC50=0.0081μg/mL.

Human C100 HEK293 cells were stained with Anti-SEMA4D / CD100 Reference Antibody (pepinemab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC231=0.3227 nM.

Pepinemab inhibited the tumor growth of CT26 on balb/c mice. The result showed significant anti-tumor effects; with an tumor inhibition rate (TGI) of 76.9% at 50 mpk at D24.