Semaphorin-4D Antibody / SEMA4D

• Catalog Number: orb749459
• Category: Antibodies
• Description: Recognizes a homodimeric protein comprised of 50kDa subunits, identified as Semaphorin-4D, or CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: A8
• Tested applications: FACS, Functional Studies, IF
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: PHA stimulated human peripheral blood lymphocytes were used as the immunogen for the Semaphorin-4D antibody.
• Dilution range: Functional studies (order BSA/sodium azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Semaphorin-4D antibody is available for research use only.
• UniProt ID: Q92854
• Storage: Store the Semaphorin-4D antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Semaphorin-4D antibody to be determined by the researcher.
• Expiration Date: 12 months from date of receipt.