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Catalog Number | orb385708 |
---|---|
Category | Antibodies |
Description | Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | SEMA4D/933 |
Tested applications | FACS, IF |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Human recombinant protein was used as the immunogen for the Semaphorin-4D antibody. |
Dilution range | Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This Semaphorin-4D antibody is available for research use only. |
UniProt ID | Q92854 |
Storage | Store the Semaphorin-4D antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Semaphorin-4D antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
FACS, Functional Studies, IF | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
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