SCYL1 Rabbit Polyclonal Antibody

SKU: orb341163

Description

The SCYL1 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human SCYL1 .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human SCYL1
• Target: SCYL1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CVAK90; GKLP; NTKL; TAPK; TEIF; TRAP; N-terminal kinase-like protein; Coated vesicle-associated kinase of 90 kDa; SCY1-like protein 1; Telomerase regulation-associated protein; Telomerase transcriptional element-interacting factor; Teratoma-associated tyrosine kinase

Western blot analysis of SCYL1 expression in Hela (A) whole cell lysates. (Predicted band size: 69; 78; 86; 88; 89 kD; Observed band size: 70 kD)

Immunohistochemical analysis of SCYL1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of SCYL1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

SCYL1

UniProt

Q96KG9

NCBI Gene ID

57410