NCC Antibody: APC

• Catalog Number: orb152699
• Category: Antibodies
• Description: Rabbit polyclonal to NCC (APC). NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis. Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC. Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension. Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat..
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC
• Reactivity: Canine, Human, Mouse, Rat
• Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:200)
• Conjugation: APC
• MW: 160kDa
• Target: NCC
• Entrez: 54300, 6559
• UniProt ID: P55017, P55018
• NCBI: NP_000330, NP_062218
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Alternative names: SLC12A3 antibody, SCYL1 antibody,CKb10 antibody, M
• Note: For research use only
• Application notes: 1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000.

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Mouse. Fixation: paraformaldehyde-fixed paraffin-embedded. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:500 for Overnight at 4°C. Secondary Antibody: Biotinylated Goat Anti-Rabbit IgG at 1:500 for 30 min at RT.

Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody. Tissue: kidney tissue. Species: Mouse. Fixation: paraformaldehyde-fixed paraffin-embedded. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000 for Overnight at 4°C. Secondary Antibody: Biotinylated Goat Anti-Rabbit IgG at 1:501 for 30 min at RT.