NCC Antibody (Biotin)

SKU: orb152700

Description

Rabbit polyclonal to NCC (Biotin). NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis. Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC. Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension. Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat..

Research Area

Cancer Biology, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IEM, IF, IHC, WB
• Dilution Range: WB (1:1000), IHC (1:200)
• Reactivity: Canine, Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
• Target: NCC
• Molecular Weight: 160kDa
• Purification: Affinity Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NCC, SLC12A3, SCYL1, CKb10, MCP-4, MGC17134, NCC-1, NCC1, SCYA13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, New CC chemokine 1, Small inducible cytokine A13, Small inducible cytokine subfamily A (Cys-Cys) member 13, Chemokine (C-C)

Quick Database Links

Gene Symbol

NCC

UniProt

P55017, P55018

NCBI Gene ID

54300, 6559

RefSeq (Protein):NP_062218, NP_000330