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Anti-RBFOX3 Antibody

Catalog Number: orb340715

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb340715
CategoryAntibodies
DescriptionRabbit polyclonal antibody to RBFOX3
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human RBFOX3
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetRBFOX3
Entrez52897, 146713
UniProt IDA6NFN3, Q8BIF2
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesRNA binding protein fox-1 homolog 3; Fox-1 homolog
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-RBFOX3 Antibody

Western blot analysis of RBFOX3 expression in A549 (A), mouse testis (B) whole cell lysates. (Predicted band size: 33; 35 kD; Observed band size: 46-55 kD)

Anti-RBFOX3 Antibody

Immunohistochemical analysis of RBFOX3 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-RBFOX3 Antibody

Immunofluorescent analysis of RBFOX3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • RBFOX3 Antibody [orb418067]

    ELISA,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • NeuN [orb736821]

    IHC-P

    Human

    Rabbit

    Monoclonal

    Unconjugated

    1 ml, 3 ml, 6 ml, 10 ml
  • RBFOX3 Antibody, HRP conjugated [orb418068]

    ELISA

    Human

    Rabbit

    Polyclonal

    HRP

    50 μg, 100 μg
  • RBFOX3 Antibody, FITC conjugated [orb418069]

    Human

    Rabbit

    Polyclonal

    FITC

    50 μg, 100 μg
  • RBFOX3 Antibody, Biotin conjugated [orb418070]

    ELISA

    Human

    Rabbit

    Polyclonal

    Biotin

    50 μg, 100 μg