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NeuN Rabbit Polyclonal Antibody

Catalog Number: orb183495

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb183495
CategoryAntibodies
DescriptionNeuN Rabbit Polyclonal Antibody
TargetRBFOX3
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Rat
Predicted ReactivityBovine, Equine, Insect, Mouse, Sheep
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human FOX3 (101-200/312aa)
UniProt IDA6NFN3
MW34 kDa
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1ug/test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesRFOX3_HUMAN; RNA binding protein fox-1 homolog 3;
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NoteFor research use only
NeuN Rabbit Polyclonal Antibody

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (NeuN) polyclonal Antibody, Unconjugated (orb183495) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

NeuN Rabbit Polyclonal Antibody

Blank control: A549. Primary Antibody (green line): Rabbit Anti-NeuN antibody (orb183495), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

NeuN Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated (orb183495) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

NeuN Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated (orb183495) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    Polyclonal

    Unconjugated

    30 μl, 100 μl, 200 μl