PI3K p85 alpha Mouse Monoclonal Antibody

SKU: orb323190

Description

The PI3K p85 alpha Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein corresponding to mouse PI3K p85 alpha. Purification: Affinity chromatography

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:1000-1:2000, IHC-P: 1:100-1:200
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant protein corresponding to mouse PI3K p85 alpha.
• Target: PIK3R1
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha

Western blot analysis of PI3K p85 alpha expression in NIH3T3 (A), rat liver (B) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 85 kD)

Immunohistochemical analysis of PI3K p85 alpha staining in rat liver, mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

PIK3R1

UniProt

Q63787, P26450

NCBI Gene ID

25513, 18708