PIK3R1 Antibody

• Catalog Number: orb395443
• Category: Antibodies
• Description: Mouse monoclonal antibody to PI3K p85 (alpha).
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: phosphoinositide-3-kinase, regulatory subunit 1(alpha)
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000-1:10000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 85 kDa
• Target: PIK3R1
• UniProt ID: P27986
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GRB1, p85, p85 ALPHA, PI 3 Kinase p85 alpha, PI 3-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wang, Weiwei et al. Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503 Artif Cells Nanomed Biotechnol, 47, 3947-3954 (2019)

PubMed: 31581849

Applications

WB

Reactivity

Rat

Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)

PubMed: 32050796

Applications

WB

Reactivity

Rat

Chen, Yanbo et al. Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways Artif Cells Nanomed Biotechnol, 47, 2179-2187 (2019)

PubMed: 31159591

Applications

WB

Reactivity

Rat

Neuro-2a cells were subjected to SDS PAGE followed by western blot with orb395443 (PIK3R1 Antibody) at dilution of 1:4000.

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using orb395443 (PIK3R1 Antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

IP Result of anti- PIK3R1 (IP: orb395443, 5 ug; Detection: orb395443 1:500) with RAW 264.7 cells lysate 1480 ug.

Immunohistochemistry of paraffin-embedded human prostate cancer slide using orb395443 (PIK3R1 Antibody) at dilution of 1:50.