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PIK3R1 Mouse Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IF, IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:10000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | phosphoinositide-3-kinase, regulatory subunit 1(alpha) |
| Target | PIK3R1 |
| Molecular Weight | 85 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−PIK3R1 Mouse Monoclonal Antibody [orb500765]
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Neuro-2a cells were subjected to SDS PAGE followed by western blot with orb395443 (PIK3R1 Antibody) at dilution of 1:4000.

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using orb395443 (PIK3R1 Antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

IP Result of anti- PIK3R1 (IP: orb395443, 5 ug; Detection: orb395443 1:500) with RAW 264.7 cells lysate 1480 ug.

Immunohistochemistry of paraffin-embedded human prostate cancer slide using orb395443 (PIK3R1 Antibody) at dilution of 1:50.
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Protocol Information
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Wang, Weiwei et al. Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503 Artif Cells Nanomed Biotechnol, 47, 3947-3954 (2019)
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Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)
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Chen, Yanbo et al. Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways Artif Cells Nanomed Biotechnol, 47, 2179-2187 (2019)
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Reactivity
PIK3R1 Mouse Monoclonal Antibody (orb395443)
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