PIK3R1 Mouse Monoclonal Antibody

• Catalog Number: orb500765
• Category: Antibodies
• Description: PIK3R1 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5C11
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant mouse PI3K p85 Protein (1-110/724aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 80 kDa
• Target: PIK3R1
• UniProt ID: P26450
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: P85A_HUMAN; Phosphatidylinositol 3-kinase regulato
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PI 3 Kinase p85 alpha) Monoclonal Antibody, Unconjugated (orb500765) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PI3K p85) Monoclonal Antibody, Unconjugated (orb500765) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: BRL-3A Cell (Rat) Lysate at 40 ug, Primary: Anti-PI3K p85 (orb500765) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 80 kD, Observed band size: 80 kD.

Sample: Lane 1: Jurkat (Human) Cell Lysate at 30 ug, Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Heart (Mouse) Lysate at 40 ug, Lane 6: Heart (Rat) Lysate at 40 ug, Lane 7: Skeletal muscle (Mouse) Lysate at 40 ug, Lane 8: Skeletal muscle (Rat) Lysate at 40 ug, Lane 9: Spleen (Mouse) Lysate at 40 ug, Lane 10: MOLT4 (Human) Cell Lysate at 30 ug, Primary: Anti-PI 3 Kinase p85 alpha (orb500765) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PI 3 Kinase p85 alpha) monoclonal Antibody, Unconjugated (orb500765) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.