Anti-PEMT Antibody

• Catalog Number: orb1880990
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PEMT
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human PEMT. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purity: The purity is > 95%
• Conjugation: Unconjugated
• Entrez: 10400, 18618
• UniProt ID: Q61907, Q9UBM1
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PEMPT; PNMT; Phosphatidylethanolamine N-methyltran
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PEMT expression in CEM (A), rat L6 (B), human liver (C) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22 kD)

Immunohistochemical analysis of PEMT staining in human hepatocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.