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PEMT Rabbit Polyclonal Antibody

Catalog Number: orb578862

DispatchUsually dispatched within 3-7 working days
$ 600.00
Catalog Numberorb578862
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PEMT
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsWB
Predicted ReactivityBovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Yeast, Zebrafish
ReactivityHuman, Rat
ImmunogenThe immunogen is a synthetic peptide directed towards the C terminal region of human PEMT
Concentration0.5 mg/ml
Form/AppearanceLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
ConjugationUnconjugated
MW22 kDa
TargetPEMT
UniProt IDQ9BW86
Protein SequenceSynthetic peptide located within the following region: GWAIMHASPTGLLLTVLVALTYIVALLYEEPFTAEIYRQKASGSHKRS
NCBINP_680477
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Alternative namesPLMT, PNMT, PEAMT, PEMPT, PEMT2
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NoteFor research use only
Expiration Date12 months from date of receipt.
PEMT Rabbit Polyclonal Antibody

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. The peptide is present in isoforms of 84, 83, 82, 71, and 42 kDa.

PEMT Rabbit Polyclonal Antibody

Sample Tissue: Human Hela Whole Cell, Antibody Dilution: 2 ug/ml.

PEMT Rabbit Polyclonal Antibody

Lanes: Lane 1: 20 ug rat liver lysate, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody Dilution: 1:15000, Gene Name: PEMT.

PEMT Rabbit Polyclonal Antibody

Lanes: Lane 1: 20 ug rat liver lysate, Primary Antibody Dilution: 1:2000, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody Dilution: 1:15000, Gene Name: PEMT.

PEMT Rabbit Polyclonal Antibody

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

PEMT Rabbit Polyclonal Antibody

WB Suggested Anti-PEMT Antibody Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate.

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