Anti-PCNA Rabbit Monoclonal Antibody

• Catalog Number: orb547082
• Category: Antibodies
• Description: Anti-PCNA Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DO-16
• Tested applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human PCNA
• Antibody Type: Primary Antibody
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 36 kDa
• UniProt ID: P12004
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Proliferating cell nuclear antigen; PCNA; Cyclin;
• Note: For research use only
• Application notes: WB 1:3000-1:10000IHC 1:50-1:200ICC/IF 1:100-1:500IP 1:50-1:100FC 1:200-1:500. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Expiration Date: 12 months from date of receipt.

WB analysis of PCNA expression in (1) HeLa Cell; (2) HepG2 Cell; (3) U937 Cell; (4) 293T Cell; (5) Mouse spleen lysate; (6) Mouse RAW264.7 Cell with PCNA Antibody.

Immunofluorescent analysis of HeLa cells, using PCNA Antibody.

Immunohistochemical analysis of paraffin-embedded human colon, using PCNA Antibody.