Anti-PCNA [SAIC-27C-101]

• Catalog Number: orb613824
• Category: Antibodies
• Description: Rabbit monoclonal antibody to PCNA
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: SAIC-27C-101
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide "LVQGSILK" derived from PCNA1 (Proliferating cellular nuclear antigen 1) conjugated to KLH. PCNA ins an auxiliary and regulatory protein of DNA polymerase delta involved in control of eukaryotic DNA replication.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: PCNA
• UniProt ID: P12004
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Proliferating cell nuclear antigen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed HeLa cells with anti-PCNA antibody SAIC-27C-101. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of SAIC-27C-101 (orb613824) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing nuclear staining. The nuclear DNA stain is DAPI (blue). Panels show from left-right, top-bottom orb613824, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.