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Anti-PCNA Antibody

Catalog Number: orb1474505

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb1474505
CategoryAntibodies
DescriptionRabbit monoclonal antibody to PCNA
Species/HostRabbit
ClonalityMonoclonal
Tested applicationsIF, IP, WB
ReactivityHuman, Mouse, Rat
ImmunogenA synthetic peptide of human PCNA
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
Form/AppearanceLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
ConjugationUnconjugated
TargetPCNA
Entrez18538, 25737, 5111
UniProt IDP17918, P12004, P04961
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
Alternative namesProliferating cell nuclear antigen; PCNA; Cyclin
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PCNA Antibody

Western blot analysis of PCNA expression in mouse spleen (A), K562 (B), C6 (C) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 29 kD)

Anti-PCNA Antibody

Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

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