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Catalog Number | orb1474505 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to PCNA |
Species/Host | Rabbit |
Clonality | Monoclonal |
Tested applications | IF, IP, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | A synthetic peptide of human PCNA |
Antibody Type | Primary Antibody |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) |
Form/Appearance | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
Conjugation | Unconjugated |
Target | PCNA |
Entrez | 18538, 25737, 5111 |
UniProt ID | P17918, P12004, P04961 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
Alternative names | Proliferating cell nuclear antigen; PCNA; Cyclin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PCNA expression in mouse spleen (A), K562 (B), C6 (C) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 29 kD)
Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |