PCNA Antibody

• Catalog Number: orb705392
• Category: Antibodies
• Description: The PCNA Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within human PCNA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human PCNA. The exact sequence is proprietary.
• UniProt ID: P12004
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:300
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Proliferating cell nuclear antigen; PCNA; Cyclin
• Research Area: Cancer, DNA Damage, Epigenetics, Infectious Diseas
• Note: For research use only
• Entrez: 5111

Western blot analysis of PCNA expression in K562 (A), HEK293 (B), A431 (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 36 kD)

Immunohistochemical analysis of PCNA staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.