PCNA Antibody

• Catalog Number: orb153410
• Category: Antibodies
• Description: Mouse monoclonal antibody to PCNA
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7H4F8
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli.
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/100 - 1/500; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 28.7kDa
• Target: PCNA
• Entrez: 5111
• UniProt ID: P12004
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of PCNA antibody

Flow cytometric analysis of MOLT4 cells lysates using PCNA antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using PCNA antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded colon cancer tissues using PCNA antibody

Western blot analysis of human PCNA using PCNA antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using PCNA antibody

Western blot analysis of A431 (Lane 1), HeLa (Lane 2), HepG2 (Lane 3), Raji (Lane 4), MOLT4 (Lane 5) cell lysate using PCNA antibody