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Catalog Number | orb197875 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to PCNA |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 7H4F8 |
Tested applications | FC, IHC, WB |
Reactivity | Human, Monkey |
Isotype | Mouse IgG1 |
Immunogen | Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli. |
Concentration | 1mg/ml |
Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
Conjugation | Unconjugated |
MW | 28.7kDa |
Target | PCNA |
Entrez | 5111 |
UniProt ID | P12004 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Ascitic fluid containing 0.03% sodium azide. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of A431 (Lane 1), HEK293 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Raji (Lane 5), MOLT4 (Lane 6), COS7 (Lane 7), MCF-7 (Lane 8) cell lysate using PCNA antibody
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of PCNA antibody
Flow cytometric analysis of MOLT4 cells lysates using PCNA antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using PCNA antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded colon cancer tissues using PCNA antibody
Western blot analysis of human PCNA using PCNA antibody
Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using PCNA antibody
FC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |