PCNA Antibody [KO/KD Validated]

SKU: orb1474010

Description

The PCNA Antibody [KO/KD Validated] is suitable for FC, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human PCNA. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/100 - 1/500), FC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant fusion protein of human PCNA. The exact sequence is proprietary.
• Purification: This antibody is purified through a protein G column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Proliferating cell nuclear antigen; PCNA; Cyclin

Western blot analysis of PCNA expression in HepG2 (A), A431 (B) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 35 kD)

Immunohistochemical analysis of PCNA staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of Hela cells using Anti-PCNA Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Quick Database Links

UniProt

P12004

NCBI Gene ID

5111