Anti-PAR4 Antibody

• Catalog Number: orb393281
• Category: Antibodies
• Description: Rabbit polyclonal antibody to F2RL3.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAR4. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500:1000, IF/ICC: 1:100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: F2RL3
• Entrez: 9002
• UniProt ID: Q96RI0
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: PAR4; Proteinase-activated receptor 4; PAR-4; Coag
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PAR4 expression in A549 (A), Panc1 (B), SGC7901 (C) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 41 kD)

Immunofluorescent analysis of PAR4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).