MET Rabbit Polyclonal Antibody

• Catalog Number: orb10422
• Category: Antibodies
• Description: MET Rabbit Polyclonal Antibody
• Target: MET
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse MET (621-720/1379aa)
• UniProt ID: P16056
• RRID: AB_10752947
• MW: 33/123/156 kDa
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MET_HUMAN; Hepatocyte growth factor receptor; EC:2
• Research Area: Cancer Research, Cell Biology, Proto-oncogenes, Re
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Stomach tissue lysates, Lane 3: Mouse Lung tissue lysates, Lane 4: Rat Kidney tissue lysates, Lane 5: Rat Stomach tissue lysates, Lane 6: Rat Lung tissue lysates, Primary: Anti-MET (orb10422) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33/123/153 kDa, Observed band size: 145 kDa.

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C-Met Polyclonal Antibody, Unconjugated (orb10422) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Tissue/Cell: human gastric cancer tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Met (c Met) Polyclonal Antibody, Unconjugated (orb10422) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.