WT1 Rabbit Polyclonal Antibody

SKU: orb329950

Description

Rabbit polyclonal antibody to WT1

Images & Validation

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Item 1 of 5

Tested Applications	IHC, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Porcine, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human WT1
Target	WT1
Molecular Weight	49 kDa
Purification	Affinity Purified

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-2310001G03Rik antibody, anti-PAR 4 antibody, anti-PAR-4 antibody, anti-Pawr antibody, anti-PAWR_HUMAN antibody, anti-PRKC Apoptosis WT1 Regulator antibody, anti-PRKC apoptosis WT1 regulator protein antibody, anti-Prostate apoptosis response 4 protein antibody, anti-Prostate apoptosis response protein 4 antibody, anti-prostate apoptosis response protein PAR-4 antibody, anti-Transcriptional repressor Par-4-like protein PAWR antibody, anti-Transcriptional repressor PAR4 antibody, anti-WT1 Interacting Protein antibody

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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The middle peptide is contained within multiple isoforms of this protein from 63 kDa to 33 kDa (~10 isoforms), and at least 4 of these isoforms seem represented in samples shown (63 kDa, 47-49 kDa, 40 kDa, and 33 kDa).

Sample Tissue: Mouse Brain, Antibody Dilution: 1 ug/mL.

Sample Tissue: Human Jurkat, Antibody Dilution: 1.0 ug/mL.

Human Testis

WB Suggested Anti-WT1 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:1562500, Positive Control: HT1080 cell lysate.

Quick Database Links

Gene Symbol

WT1

UniProt

P19544

RefSeq (Protein):NP_077742

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_077742

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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