P53/TP53 Rabbit Polyclonal Antibody

SKU: orb180706

Description

P53/TP53 Rabbit Polyclonal Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human P53 recombinant protein (Position: A74-D393). Human P53 shares 83% and 85% amino acid (aa) sequences identity with mouse and rat P53, respectively.
• Target: Cellular tumor antigen p53
• Molecular Weight: 53 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53

Flow Cytometry analysis of CACO-2 cells using anti-P53 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of P53 using anti-P53 antibody.Lane 1:human CACO-2 cell; 2:human HepG2 cell; 3:human A431 cell.

IF analysis of P53 using anti-P53 antibody. P53 was detected in immunocytochemical section of A431 cells.

IHC analysis of P53 using anti-P53 antibody. P53 was detected in paraffin-embedded section of human ovarian cancer tissue.

IHC analysis of P53 using anti-P53 antibody. P53 was detected in paraffin-embedded section of human lung cancer tissue.

Quick Database Links

Gene Symbol

Cellular tumor antigen p53

UniProt

P04637