Anti-P53/TP53 Antibody

• Catalog Number: orb180706
• Category: Antibodies
• Description: Anti-P53/TP53 Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E.coli-derived human P53 recombinant protein (Position: A74-D393). Human P53 shares 83% and 85% amino acid (aa) sequences identity with mouse and rat P53, respectively.
• UniProt ID: P04637
• MW: 53 kDa
• Tested applications: FC, ICC, IF, IHC, WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Cross Reactivity: No cross-reactivity with other proteins
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cellular tumor antigen p53; Antigen NY-CO-13; Phos
• Note: For research use only

Flow Cytometry analysis of CACO-2 cells using anti-P53 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of P53 using anti-P53 antibody.Lane 1:human CACO-2 cell; 2:human HepG2 cell; 3:human A431 cell.

IF analysis of P53 using anti-P53 antibody. P53 was detected in immunocytochemical section of A431 cells.

IHC analysis of P53 using anti-P53 antibody. P53 was detected in paraffin-embedded section of human ovarian cancer tissue.

IHC analysis of P53 using anti-P53 antibody. P53 was detected in paraffin-embedded section of human lung cancer tissue.