p53 Antibody (S315)

• Catalog Number: orb1930141
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB7777
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 43653 Da
• Target: This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from human p53.
• UniProt ID: P04637
• NCBI: NP_001119590.1, NP_001119585.1, NP_001119586.1, NP_001119584.1, NP_001119589.1, NP_001263625.1, NP_001263626.1, NP_000537.3, NP_001263628.1, NP_00126, NP_001119587.1, NP_001263624.1, NP_001119588.1, NP_001263627.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cellular tumor antigen p53, Antigen NY-CO-13, Phos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of U251 cell stained with p53 Antibody (S315).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with p53 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). p53 immunoreactivity is localized to Nucleus significantly.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with p53 Antibody (S315), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

p53 Antibody (S315) western blot analysis in Daudi cell line lysates (35 ug/lane). This demonstrates the p53 antibody detected the p53 protein (arrow).

All lanes: Anti-p53 Antibody (S315) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HT-29 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-p53 Antibody (S315) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: HT-29 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-p53 Antibody (S315) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HT-29 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-p53 Antibody (S315) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.