Anti-Orexin Receptor 1 Antibody

• Catalog Number: orb1289807
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to Orexin Receptor 1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Orexin Receptor 1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:1000, IF:IC: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HCRTR1
• Entrez: 3061, 230777, 25593
• UniProt ID: P58307, O43613, P56718
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Orexin receptor type 1; Ox-1-R; Ox1-R; Ox1R; Hypoc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Orexin Receptor 1 expression in U87MG (A), rat brain (B), mouse brain (C) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 55 kD)

Immunofluorescent analysis of Orexin Receptor 1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.