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CD3 Antibody (Biotin)
SKU: orb179872
Description
Research Area
Cancer, Epigenetics, Immunology
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC, IHC-Fr, IP |
|---|---|
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Armenian Hamster IgG kappa |
| Clone No. | 145-2C11 |
| Immunogen | Mouse BM10-37 cytotoxic T lymphocytes |
| Target | CD3 |
| Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store at 2-8°C. Do not freeze. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CD3E, T3E, TCRE
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Separation of murine CD3 positive splenocytes (red-filled) from CD3 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) Biotin antibody (concentration in sample 8 μg/ml, Streptavidin APC).
Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) Biotin antibody (concentration in sample 8 μg/ml, Streptavidin APC).
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Protocol Information
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
CD3 Antibody (Biotin) (orb179872)
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