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CD3 Antibody (Biotin)
SKU: orb222827
Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, IHC-Fr, IP |
|---|---|
| Reactivity | Human, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Clone No. | UCHT1 |
| Immunogen | human thymocytes followed by Sezary T cells |
| Target | CD3 |
| Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store at 2-8°C. Do not freeze. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CD3E, T3E, TCRE
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Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (UCHT1) biotin antibody (concentration in sample 0.3 μg/ml) streptavidin APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (UCHT1) biotin antibody (concentration in sample 0.3 μg/ml) streptavidin APC.
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Protocol Information
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IP
Immunoprecipitation
CD3 Antibody (Biotin) (orb222827)
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