CD3 Antibody (Biotin)

• Catalog Number: orb2704159
• Category: Antibodies
• Description: CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
• Target: CD3
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Conjugation: Biotin
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• UniProt ID: P07766
• Tested applications: FC, ICC
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Clone Number: TB3
• Storage: Store at 2-8°C. Do not freeze.
• Alternative names: CD3E, T3E, TCRE
• Note: For research use only
• Entrez: 916
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (TB3) biotin antibody (concentration in sample 0.56 µg/ml, Streptavidin APC).

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (TB3) biotin antibody (concentration in sample 0.56 µg/ml, Streptavidin APC).