Microtubule Associated Protein 2 (MAP2) Antibody

SKU: orb319566

Description

Our Anti-Microtubule Associated Protein 2 (MAP2) primary antibody from Biorbyt is chicken polyclonal. It detects bovine, human, mouse, and rat Microtubule Associated Protein 2 (MAP2) and is total IgY fraction.

Research Area

Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 1:20000, IHC: 1:2500-1:10,000, ICC: 1:1000-1:20,000
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Gallus
• Clonality: Polyclonal
• Isotype: IgY
• Immunogen: Recombinant bovine MAP2 protein expressed in and purified from E. Coli.
• Target: Microtubule Associated Protein 2 (MAP2)
• Molecular Weight: 280
• Purification: Total IgY fraction
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 10 mM NaN3.
• Concentration: 19 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DKFZp686I2148 antibody, MAP 2 antibody, MAP dendrite specific antibody, MAP-2 antibody, MAP2 antibody, MAP2A antibody, MAP2B antibody, MAP2C antibody, Microtubule associated protein 2 antibody, Microtubule-associated protein 2 antibody, MTAP2_HUMAN antibody

(A-B). Primary hippocampal neurons were stained with MAP2 (Cat. orb319566, 1:1000) as a negative control under basal conditions (CTRL) and with picrotoxin treatment (PTX).

Costaining of the primary brain cells isolated from 18-day embryonic rats (E18). Neurons stained with α-MAP2 (green). Copurified astrocytes stained with α-GFAP (red).

Day 14 (D14) RGC-iNs stained with a DAPI, TAU, and MAP2 (Cat. orb319566, 1:1000).

Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 (Cat. orb319566, red, 1:5000) and Anti-FOX3 (Cat. orb613671 , green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.

Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2 (Cat. orb319566, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.

Immunofluorescence of rat cerebellum showing strong synaptic staining of VSNL1 (Cat. : orb319709, green) in the molecular layer (ML) and MAP2 (Cat. orb319566, red, 1:2500). The blue stain is nuclear DNA.

Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (Cat. orb319549, 1:1000, green) and anti-MAP2 (orb319566, 1:1000, red).

Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (Cat. orb319549, 1:1000, green) and anti-MAP2 (orb319566, 1:1000, red).

Immunolabeling of mouse hippocampal cultures labeled with anti-MAP2 (Cat. orb319566, 1:1000, red) and anti-synapsin (Cat. orb319666 , 1:1000, green).

Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (Cat. orb319583, red, 1:500) and anti-MAP2 antibody (Cat. orb319566, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.

Immunostaining of rat hippocampus showing specific immunolabeling of MAP2 (Cat. orb319566, red, 1:1000), Tau-1 (green), and nuclear DNA labeled with DAPI (blue).

Representative 3D view images of paired helical filament (PHF)-tau-treated human brain organoid fractions. The organoids were labeled for MAP2 (Cat. orb319566, 1:1000, green), NR3A (red), PSD95 (magenta), and DAPI (blue). White arrows show the positions colocalized between PSD95 and NR3A. The dotted circles show the magnified positions. Scale bar = 10 or 3 μm.

Representative images of anti-MAP2 (ca. orb319566) immunolabeled rat neurons at 5 DIV of T30 treatment.

Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.

Quick Database Links

Gene Symbol

Microtubule Associated Protein 2 (MAP2)

UniProt

P11137