ERK1 + ERK2 Rabbit Polyclonal Antibody

• Catalog Number: orb6018
• Category: Antibodies
• Description: ERK1 + ERK2 Rabbit Polyclonal Antibody
• Target: MAPK3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Goat, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human ERK1/2 (251-358/358aa)
• UniProt ID: P27361
• RRID: AB_10922098
• MW: 42/44 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1 μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERK1/2; ERK1+ERK2; ERK1/ERK2; ERK 1; ERK 2; ERK-2;
• Research Area: Cell Biology, Cytoplasmic, MAPK/ERK Signalling Pat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhao, Haiyan et al. Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension Int Immunopharmacol, 42, 115-121 (2017)

PubMed: 27912147

Zhang, Fengwei et al. Seasonal Expression of Oxytocin and Oxytocin Receptor in the Scented Gland of Male Muskrat (Ondatra zibethicus) Sci Rep, 7, 16627 (2017)

PubMed: 29192229

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (orb6018) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: Lymph node (Mouse) Lysate at 40 ug, Lane 5: Lymph node (Rat) Lysate at 40 ug, Lane 6: SiHa (Human) Cell Lysate at 30 ug, Lane 7: HUVEC (Human) Cell Lysate at 30 ug, Primary: Anti-ERK1 + ERK2 (orb6018) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44'42 kD, Observed band size: 38 kD.

Tissue/Cell: HUVEC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (orb6018) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: HUVEC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (orb6018) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.