Anti-Mesothelin [MB]

SKU: orb1821533

Description

Anti-Mesothelin [MB]

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC, SBR, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: MB
• Immunogen: The original antibody was generated in mesothelin-deficient mice by immunization with plasmid cDNA corresponding to the mesothelin extracellular domain followed by a single boost with a recombinant human mesothelin-rabbit IgG Fc fusion protein.
• Target: Mesothelin
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; MN

Flow cytometry using anti-Mesothelin antibody MB. HeLa cells were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of MB (orb1821533, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-Mesothelin antibody MB. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MB (orb1821533) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821533, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-Mesothelin antibody MB. HeLa cells lysate (35 µg protein in RIPA buffer) was resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of MB (orb1821533) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

Mesothelin

UniProt

Q13421