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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1.430555556, IHC: 1:500-1:5000, ICC: 1:1000-1:5000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 5H12 |
| Immunogen | Full length human recombinant MeCP2 |
| Target | MeCP2 |
| Molecular Weight | 75 |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS + 50% glycerol and 5 mM NaN3 |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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ELISA, FC, ICC, IF, IHC, WB
Human, Mouse, Rat
Rabbit
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100 μgHuman Methyl CpG Binding Protein 2 (MECP2) ELISA Kit [orb778292]
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0.32-20 ng/mL
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Immunofluorescence of a section of rat cerebellum labeled with anti-MeCP2 (Cat. orb55908, 1:500, red), colabeled with anti-GFAP (Cat. orb319549, 1:5000, green), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.

Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (Cat. orb55908, 1:1000, red) in nuclei of neurons and specific labeling of GFAP (Cat. orb319550, 1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.

Immunostaining of a section of rat hippocampus showing specific nuclear immunolabeling of MeCP2 (Cat. orb55908, red, 1:1000) and FOX3 (green). The blue is DAPI staining of nuclear DNA.

Western blot of mouse whole brain lysate showing specific immunolabeling of the MeCP2 protein at ~75 kDa.
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MeCP2 Antibody (orb55908)
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