MeCP2 Antibody (N-term S80)

SKU: orb1937821

Description

MeCP2 Antibody (N-term S80)

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 52441 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-MeCP2 Antibody (N-term S80) at 1:500 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

MeCP2 Antibody (N-term S80) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MeCP2 Antibody (N-term S80) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MeCP2 Antibody (N-term S80) for immunohistochemistry. Clinical relevance has not been evaluated.

MeCP2 Antibody (pS80) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).

Quick Database Links

UniProt

P51608