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Item 1 of 4
Phospho-MeCP2(S423) Antibody
Catalog Number: orb1931066
| Catalog Number | orb1931066 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This MeCP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S423 of human MeCP2. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | P51608 |
| MW | 52441 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:500, WB: 1:500, WB: 1:500, IHC-P-Leica: 1:500 |
| Antibody Type | Primary Antibody |
| Clone Number | RB29261 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp Read more... |
| Note | For research use only |
| NCBI | NP_001104262.1, NP_004983.1 |
Immunohistochemical analysis of paraffin-embedded Human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Western blot analysis of lysates from C6 cell line, untreated or treated with calf intestinal alkaline phosphatase (CIP), using Phospho-MeCP2 (S423) Antibody (upper) or Beta-actin (lower).
Western blot analysis of lysates from Jurkat cell line, untreated or treated with calf intestinal alkaline phosphatase (CIP), using Phospho-MeCP2 (S423) Antibody (upper) or Beta-actin (lower).
Western blot analysis of lysates from Neuro-2a cell line, untreated or treated with calf intestinal alkaline phosphatase (CIP), using Phospho-MeCP2 (S423) Antibody (upper) or Beta-actin (lower).
